PROJECT REPORT No. 335 DEVELOPMENT OF A BIOSENSOR ARRAY TO RAPIDLY DETECT AND MEASURE ORGANOPHOSPHATE PESTICIDES IN GRAIN

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چکیده

......................................................................................................................................................................... 1 SUMMARY........................................................................................................................................................................... 2 INTRODUCTION ................................................................................................................................................................... 2 PROJECT OVERVIEW............................................................................................................................................................ 5 CONCLUSIONS..................................................................................................................................................................... 7 TECHNICAL DETAIL 1 SELECTION AND STABILIZATION OF ENZYMES.................................................. 8 INTRODUCTION ................................................................................................................................................................... 8 INITIAL STABILIZER SCREENS: ............................................................................................................................................ 8 SELECTION OF DROSOPHILA ACHE MUTANTS. .................................................................................................................. 8 STABILIZER SCREENS ON ACHE MUTANTS......................................................................................................................... 9 STABILIZER SCREENS ON CARBON SQUARES .................................................................................................................... 10 LONG TERM STABILITY OF ACHE BASED SENSORS .......................................................................................................... 12 CONCLUSIONS................................................................................................................................................................... 13 TECHNICAL DETAIL 2 RAPID EXTRACTION AND OXIDATION OF ORGANOPHOSPHATE PESTICIDE RESIDUES FROM WHEAT AND APPLES........................................................................................... 14 INTRODUCTION ................................................................................................................................................................. 14 MATERIALS....................................................................................................................................................................... 14 APPARATUS ...................................................................................................................................................................... 15 EXTRACTION VESSEL ....................................................................................................................................................... 15 METHODS.......................................................................................................................................................................... 16 RESULTS ........................................................................................................................................................................... 17 CONCLUSIONS................................................................................................................................................................... 19 REFERENCES ..................................................................................................................................................................... 20 TECHNICAL DETAIL 3 DEVELOPMENT OF BIOSENSORS AND PATTERN RECOGNITION SOFTWARE ....................................................................................................................................................................... 2

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تاریخ انتشار 2004